Leptospirosis serosurvey in bovines from Brazilian Pantanal using IGG ELISA with recombinant protein LipL32 and microscopic agglutination test
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منابع مشابه
Leptospirosis Serosurvey in Bovines from Brazilian Pantanal Using Igg Elisa with Recombinant Protein Lipl32 and Microscopic Agglutination Test
This investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region’s climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutinati...
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BACKGROUND Leptospirosis is diagnosed on clinical grounds, and confirmed by microscopic agglutination test (MAT). IgM-ELISA (Serion-Virion) and immunochromatography test (Leptocheck-WB) are two immunodiagnostic assays for leptospirosis. Their sensitivity, specificity and applicability in Sri Lanka have not been systematically evaluated. METHODS Clinically diagnosed leptospirosis patients (n =...
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Leptospirosis is worldwide zoonotic disease that is serious problem in south Indian states and now emerging in North India. The focus these days is to develop recombinant outer membrane protein based Enzyme-linked immunosorbent assay (ELISA) for seroprevalence of leptospirosis. The aim of this study was to evaluate indirect ELISA based on recombinant LipL41, Loa22, LipL21, LipL32 and OmpL1 prot...
متن کاملEvaluation of the recombinant LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis of bovine leptospirosis.
The recombinant leptospiral protein LipL32 was evaluated for use in the diagnosis of bovine leptospirosis by enzyme-linked immunosorbent assay (rLipL32 IgG ELISA). The microscopic agglutination test (MAT) of 150 serum samples from cattle suspected of leptospirosis showed that 125 (83.3%) samples had positive reciprocal agglutination titres, which ranged from 100 to 1600. The highest titres were...
متن کاملAssessment of the efficacy of an IgM-elisa and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis.
In a prospective study in Barbados between 1979 and 1989, 321 cases were diagnosed in 638 patients presenting at a hospital with symptoms of leptospirosis. Initial diagnosis was based on patient history and characteristic signs and symptoms. In 92 cases (29%), diagnosis was confirmed by isolation of organisms from the blood, urine, or dialysate fluid; in the remaining 229 cases (71%) diagnosis ...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2007
ISSN: 1517-8382
DOI: 10.1590/s1517-83822007000400017